deCIPher
Sample Information Sequencing data Molecular Characterization Homology Insertion Site1 Insertion Site2 Insertion Site3

Report - Basic Information

Sample Information

SpeciesSoybean
Genome Size978.4 Mb
Chromosome Count20
Insert Gene Size7303
Project ID68eefe98-2434-4f6f-8ea8-611c671ef4d7
Submission Time2024-10-23 06:53:44
Report Time2024-10-23 07:21:08
Runtime1644.63s

Diagram of chromosomes

Sequencing Data

Sequencing TypeIllumina
Pre QC Size1638 Mb
Post QC Size1638 Mb
Coverage1100.00%
Coverage1039.90%
Coverage Half Depth62.88%

Diagram of coverage distribution

coverage_distribution

Molecular Characterization

Genome Depth11.82
Genome Standard Deviation15.56
T-DNA Depth100.79
T-DNA Standard Deviation49.76
T-DNA Copy Number8.523

Backbone Residue

residue

Report - Insertion Sites

Homologous Analysis

Top 10 BLAST Alignments

Number Chromosome Genome Start Genome End T-DNA Start T-DNA End Score Identity (%) Alignment Length
Homology Value = Total Aligned Bases T-DNA Length × 100 %

TDNA BLAST Frequency

tdna_depth

Insertion Region:('chromosome_15', 13710333, 13711449)

Specific Positionchromosome_15: 13710949
Site in T-DNA2293
Directionforward
Order of BoundaryUpstream
Unexpected Insertion-2 bp
Full Sequence of Small Insertion

Diagram of insertion site

insertion_site1-1

Flanking sequences:

( genome sequence    T-DNA sequence)
ATTCAAGTTTTTACAAAATACTTAAGCTTCTAGAAATTATAATTATCTTCTACATATTTTCATCCTTTGAAAATATTTAATGACTTTTTTGTTGTCATTAATACTATAATCGACAT GGCAGTGATAGCCACTAAGAATCGTCTTTTGTTTTACATGTGGCGCCACAAATTAGGGTAATGAAGCGGCAATATTTTGGAACTCGGAAAATAAAATTGCGCCATCACATTATTTGAAAATTTTCACATGCTTTTATTTTAAAAACCCACGAATTACAAGTAAAATAAGGGAAACTAAACAATGGAACATACAATTTAAATTAGCGCAGAAGATTGATTGGAATAGTCACTTGTAAGTCGAGCTACAGGGTTGATATTGTCTTTGAAGGTGAATTG


Specific Positionchromosome_15: 13710949
Site in T-DNA2624
Directionforward
Order of BoundaryUpstream
Unexpected Insertion-2 bp
Full Sequence of Small Insertion

Diagram of insertion site

insertion_site1-2

Flanking sequences:

( genome sequence    T-DNA sequence)
ATTCAAGTTTTTACAAAATACTTAAGCTTCTAGAAATTATAATTATCTTCTACATATTTTCATCCTTTGAAAATATTTAATGACTTTTTTGTTGTCATTAATACTATAATCGACAT GGCAGTGATAGCCACTAAGAATCGTCTTTTGTTTTACATGTGGCGCCACAAATTAGGGTAATGAAGCGGCAATATTTTGGAACTCGGAAAATAAAATTGCGCCATCACATTATTTGAAAATTTTCACATGCTTTTATTTTAAAAACCCACGAATTACAAGTAAAATAAGGGAAACTAAACAATGGAACATACAATTTAAATTAGCGCAGAAGATTGATTGGAATAGTCACTTGTAAGTCGAGCTACAGGGTTGATATTGTCTTTGAAGGTGAATTG


Specific Positionchromosome_15: 13710949
Site in T-DNA4111
Directionreverse
Order of BoundaryUpstream
Unexpected Insertion-2 bp
Full Sequence of Small Insertion

Diagram of insertion site

insertion_site1-3

Flanking sequences:

( genome sequence    T-DNA sequence)
ATTCAAGTTTTTACAAAATACTTAAGCTTCTAGAAATTATAATTATCTTCTACATATTTTCATCCTTTGAAAATATTTAATGACTTTTTTGTTGTCATTAATACTATAATCGACAT GGCAGTGATAGCCACTAAGAATCGTCTTTTGTTTTACATGTGGCGCCACAAATTAGGGTAATGAAGCGGCAATATTTTGGAACTCGGAAAATAAAATTGCGCCATCACATTATTTGAAAATTTTCACATGCTTTTATTTTAAAAACCCACGAATTACAAGTAAAATAAGGGAAACTAAACAATGGAACATACAATTTAAATTAGCGCAGAAGATTGATTGGAATAGTCACTTGTAAGTCGAGCTACAGGGTTGATATTGTCTTTGAAGGTGAATTG


Insertion Region:('chromosome_15', 15115505, 15116736)

Specific Positionchromosome_15: 15116094
Site in T-DNA7263
Directionreverse
Order of BoundaryUpstream
Unexpected Insertion24 bp
Full Sequence of Small InsertionTAACAAAAAGACGGATGAACCACA

Diagram of insertion site

insertion_site2-1

Flanking sequences:

( genome sequence    T-DNA sequence)
CGTCGAAATGACATTGAGATTGATGGAGCGTTTTGGTGTGAAAGCAGAGCATTCTGATAGCTGGGACAGATTCTACATTAAGGGAGGTCAAAAATACAAGTCCCCTAAAAATGCCTATGTTGAAGGTGATGCCTCAAGCGCAAGCTATTTCTTGGCTGGTGCTGCAATTACTGGAGGGACTGTGACTGTGGAAGGTTGTGGCACCACCAGTTTGCAGGGTGATGTGAAGTTTGCTGAGGTACTGGAGATGATGGGAGCGAAGGTTACATGGACCGAGACTAGCGTAACTGTTACTGGCCCACCGCGGGAGCCATTTGGGAGGAAACACCTCAAGGCGATTGATGTCAACATGAACAAGATGCCTGATGTCGCCATGACTCTTGCTGTGGTTGCCCTCTTTGCCGATGGCCCGACAGCCATCAGAGACGTGGCTTCCTGGAGAGTAAAGGAGACCGAGAGGATGGTTGCGATCCGGACGGAGCTAACCAAGCTGGGAGCATCTGTTGAGGAAGGGCCGGACTACTGCATCATCACGCCGCCGGAGAAGCTGAACGTGACGGCGATCGACACGTACGACGACCACAGGATGGCGATGGCTTTCTCCCTTGCCGCCTGTGCCGAGGTCCCCGTCACCATCCGGGACCCTGGGTGCACCCGGAAGACCTTCCCCGACTACTTCGATGTGCTGAGCACTTTCGTCAAGAATTAAGCTCTAGAACTAGTGGATCCCCCGATCCGCGTTTGTGTTTTCTGGGTTTCTCACTTAAGCGTCTGCGTTTTACTTTTGTATTGGGTTTGGCGTTTAGTAGTTTGCGGTAGCGTTCTTGTTATGTGTAATTACGCTTTTTCTTCTTGCTTCAGCAGTTTCGGTTGAAATATAAATCGAATCAAGTTTCACTTTATCAGCGTTGTTTTAAATTTTGGCATTAAATTGGTGAAAATTGCTTCAATTTTGTATCTAAATAGAAGAGACAACATGAAATTCGACTTTTGACCTCAAATCTTCGAACATTTATTTCCTGATTTCACGATGGATGAGGATAACGAAAGGGCGGTTCCTATGTCCGGGAAAGT TCCCGTAGAAGACAATGAGCAAAGCTACTGAAACGCGGACACGACGTCGCATTGGTACGGATATGAGTTAAACCGACTCAATTCCTTTATTAAGACATAAACCGATTTTGGTTAAAGTGTAACAGTGAGCTGATATAAAACCGAAACAAACCGGTACAAGTTTGATTGAGCAACTTGATGACAAACTTCAGAATTTTGGTTATTGAATGAAAATCATAGTCTAATCGTAAAAAATGTACAGAAGAAAAGCTAGAGCAGAACAAAGATTCTATATTCTGGTTCCAATTTATCATCGCTTTAACGTCCCTCAGATTTGATCGGGCTGCAGGAATTAATGTGGTTCATCCGTCTTTTTGTTAATGCGGTCATCAATACGTGCCTCAAAGATTGCCAAATAGATTAATGTGGTTCATCTCCCTATATGTTTTGCTTGTTGGATTTTGCTATC


Specific Positionchromosome_15: 15116119
Site in T-DNA2787
Directionforward
Order of BoundaryDownstream
Unexpected Insertion-3 bp
Full Sequence of Small Insertion

Diagram of insertion site

insertion_site2-2

Flanking sequences:

( genome sequence    T-DNA sequence)
ATTCAAGTTTTTACAAAATACTTAAGCTTCTAGAAATTATAATTATCTTCTACATATTTTCATCCTTTGAAAATATTTAATGACTTTTTTGTTGTCATTAATACTATAATCGACATGGCAGTGATAGCCACTAAGAATCGTCTTTTGTTTTACATGTGGCGCCACAAATTAGGGTAATGAAGCGGCAATATTTTGGAACTCGGAAAATAAAATTGCGCCATCACATTATTTGAAAATTTTCACATGCTTTTATTTTAAAAACCCACGAATTACA AGTAAAATAAGGGAAACTAAACAATGGAACATACAATTTAAATTAGCGCAGAAGATTGATTGGAATAGTCACTTGTAAGTCGAGCTACAGGGTTGATATTGTCTTTGAAGGTGAATTG


Specific Positionchromosome_15: 15116119
Site in T-DNA2456
Directionforward
Order of BoundaryDownstream
Unexpected Insertion-3 bp
Full Sequence of Small Insertion

Diagram of insertion site

insertion_site2-3

Flanking sequences:

( genome sequence    T-DNA sequence)
ATTCAAGTTTTTACAAAATACTTAAGCTTCTAGAAATTATAATTATCTTCTACATATTTTCATCCTTTGAAAATATTTAATGACTTTTTTGTTGTCATTAATACTATAATCGACATGGCAGTGATAGCCACTAAGAATCGTCTTTTGTTTTACATGTGGCGCCACAAATTAGGGTAATGAAGCGGCAATATTTTGGAACTCGGAAAATAAAATTGCGCCATCACATTATTTGAAAATTTTCACATGCTTTTATTTTAAAAACCCACGAATTACA AGTAAAATAAGGGAAACTAAACAATGGAACATACAATTTAAATTAGCGCAGAAGATTGATTGGAATAGTCACTTGTAAGTCGAGCTACAGGGTTGATATTGTCTTTGAAGGTGAATTG


Specific Positionchromosome_15: 15116119
Site in T-DNA3948
Directionreverse
Order of BoundaryDownstream
Unexpected Insertion-3 bp
Full Sequence of Small Insertion

Diagram of insertion site

insertion_site2-4

Flanking sequences:

( genome sequence    T-DNA sequence)
ATTCAAGTTTTTACAAAATACTTAAGCTTCTAGAAATTATAATTATCTTCTACATATTTTCATCCTTTGAAAATATTTAATGACTTTTTTGTTGTCATTAATACTATAATCGACATGGCAGTGATAGCCACTAAGAATCGTCTTTTGTTTTACATGTGGCGCCACAAATTAGGGTAATGAAGCGGCAATATTTTGGAACTCGGAAAATAAAATTGCGCCATCACATTATTTGAAAATTTTCACATGCTTTTATTTTAAAAACCCACGAATTACA AGTAAAATAAGGGAAACTAAACAATGGAACATACAATTTAAATTAGCGCAGAAGATTGATTGGAATAGTCACTTGTAAGTCGAGCTACAGGGTTGATATTGTCTTTGAAGGTGAATTG


Insertion Region:('chromosome_15', 16942510, 16943626)

Specific Positionchromosome_15: 16943010
Site in T-DNA6956
Directionforward
Order of BoundaryUpstream
Unexpected Insertion0 bp
Full Sequence of Small Insertion

Diagram of insertion site

insertion_site3-1

Flanking sequences:

( genome sequence    T-DNA sequence)
CGAAAATTTATTTACTGATTTCACGTTTGATGAGGATAACGATAGGGCGGTTCCTATGTACGGGAAAGTTCCCTTAGAAGACAATGAGCAAAGCTAC ATAAATTTAATATAATTTATAATATGATAAAAAAATAAAAATATTAATAGGAGATTTAAAATAAATAATAAG