PubMed 25277299

PubMed ID: 25277299

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Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts.
Authors: Ichinose Mizuho, Uchida Masato, Sugita Mamoru
Journal: FEBS letters (FEBS Lett), Vol.588(21), 2014‑Nov‑03

DOI: 10.1016/j.febslet.2014.09.031

Abstract
The moss Physcomitrella patens has two RNA editing sites in the chloroplasts. Here we identified a novel DYW-subclass pentatricopeptide repeat (PPR) protein, PpPPR_45, as a chloroplast RNA editing factor in P. patens. Knockdown of the PpPPR_45 gene reduced the extent of RNA editing at the chloroplast rps14-C2 site, whereas over-expression of PpPPR_45 increased the levels of RNA editing at both the rps14-C2 site and its neighboring C site. This indicates that the expression level of PpPPR_45 affects the extent of RNA editing at the two neighboring sites.
Publication Types
Journal Article Research Support, Non-U.S. Gov't
Keywords
Chloroplast DYW domain Pentatricopeptide repeat protein Physcomitrella patens RNA editing
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