High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9.
Authors: Chen Yudong, Xiang Haiying, Jia Ling, Yang Qianxu, Zhang Jianduo, Jiang Jiarui, Zeng Wanli, Deng Lele, Jin Jingjing, Gao Qian, Li Xuemei
Journal: Plant cell reports (Plant Cell Rep), Vol.42(12), 2023‑Dec
DOI:
10.1016/j.molp.2017.06.006
PMCID:
5543939
Abstract
4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.
Publication Types
Journal Article
Grant Support
- Creation and Identification of Editing Material Library of Tobacco Transporter and Enzyme Related Genes in Red flower Mammoth Gold (): 110202101034
- Development of Tagless Gene Editing Technology and Breeding Application of Low Nicotine Editing Materials (): 2022JY03
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