Function Introduction

This module allows users to analyze sgRNA efficiency and off-target risks across various plant genomes. By selecting a species, entering the sgRNA sequence, and setting a mismatch threshold (0–4), users can predict off-target sites and assess editing precision. Results include off-target alignments, MIT, CFD, and CRISPRscan scores, plus mutation heatmaps and nucleotide distributions, supporting optimized sgRNA design and risk evaluation for plant genome editing.