Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele.
Authors: Tomlinson Laurence, Yang Ying, Emenecker Ryan, Smoker Matthew, Taylor Jodie, Perkins Sara, Smith Justine, MacLean Dan, Olszewski Neil E, Jones Jonathan D G
Journal: Plant biotechnology journal (Plant Biotechnol J), Vol.17(1), 2019‑Jan
DOI:
10.1093/jxb/ert022
PMCID:
PMC1913748
Abstract
The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.
Publication Types
Journal Article
Research Support, Non-U.S. Gov't
Keywords
CRISPR/Cas9
DELLA
PROCERA
dwarf
gibberellic acid
slender
Grant Support
- Gatsby Charitable Foundation (International):
- Biotechnology and Biological Sciences Research Council (United Kingdom): BB/L009293/1
- US Israel Binational Agriculture Research and Development Fund (International): US-4813-15C
- Biotechnology and Biological Sciences Research Council (BBSRC) (International): BB/L009293/1
- Bill and Melinda Gates Foundation (International): OPP1060026
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