Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM.
Authors: Endo Masaki, Mikami Masafumi, Endo Akira, Kaya Hidetaka, Itoh Takeshi, Nishimasu Hiroshi, Nureki Osamu, Toki Seiichi
Journal: Nature plants (Nat Plants), Vol.5(1), 2019‑Jan
DOI:
10.1186/1746-4811-5-16
Abstract
Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1) can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.
Publication Types
Journal Article
Research Support, Non-U.S. Gov't
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