Improving CRISPR-Cas-mediated RNA targeting and gene editing using SPLCV replicon-based expression vectors in Nicotiana benthamiana.
Authors: Yu Yicheng, Wang Xiao, Sun Houjun, Liang Qiang, Wang Weichi, Zhang Chengling, Bian Xiaofeng, Cao Qinghe, Li Qiang, Xie Yiping, Ma Daifu, Li Zongyun, Sun Jian
Journal: Plant biotechnology journal (Plant Biotechnol J), Vol.18(10), 2020‑Oct
DOI:
10.1111/pbi.13384
PMCID:
PMC6161460
Abstract
【无摘要】
Publication Types
Journal Article
Keywords
Nicotiana benthamiana
LbCas12a
LwaCas13a
RNA targeting and gene editing efficiency
SpCas9
deconstructed SPLCV strategy
Grant Support
- the National Key R & D Program of China (): 2018YFD1000703, 2018YFD1000700
- the earmarked fund for China Agriculture Research System (): CARS-10-B03
- the National Natural Science Foundation of China (): 31871684
- the Key R & D Program of Xuzhou (): KC19127
- the Priority Academic Program Development of Jiangsu Higher Education Institutions (): PAPD
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