Targeted mutagenesis in Arabidopsis thaliana using CRISPR-Cas12b/C2c1.
Authors: Wu Fan, Qiao Xinyu, Zhao Yafei, Zhang Ziyi, Gao Yifan, Shi Lingfeng, Du Haokun, Wang Lulu, Zhang Ya-Jie, Zhang Yu, Liu Langyu, Wang Quan, Kong Dejing
Journal: Journal of integrative plant biology (J Integr Plant Biol), Vol.62(11), 2020‑Nov
DOI:
10.1111/jipb.12944
Abstract
Cas12b/C2c1 is a newly identified class 2 CRISPR endonuclease that was recently engineered for targeted genome editing in mammals and rice. To explore the potential applications of the CRISPR-Cas12b system in the dicot Arabidopsis thaliana, we selected BvCas12b and BhCas12b v4 for analysis. We successfully used both endonucleases to induce mutations, perform multiplex genome editing, and create large deletions at multiple loci. No significant mutations were detected at potential off-target sites. Analysis of the insertion/deletion frequencies and patterns of mutants generated via targeted gene mutagenesis highlighted the potential utility of CRISPR-Cas12b systems for genome editing in Arabidopsis.
Publication Types
Journal Article
Grant Support
- National Natural Science Foundation of China (): 31570190
- National Natural Science Foundation of China (): 31800224
- Science Technology and Innovation Committee of Shenzhen Municipality (): JCYJ20170303154319837
- Science Technology and Innovation Committee of Shenzhen Municipality (): JCYJ20170412155447658
- Natural Science Foundation of Hebei Province (): C2017208087
- Science and Technology Research Program for Colleges and Universities in Hebei Province (): QN2018149
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