Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange.
Authors: Tang Xiaomei, Chen Shulin, Yu Huiwen, Zheng Xiongjie, Zhang Fei, Deng Xiuxin, Xu Qiang
Journal: Plant cell reports (Plant Cell Rep), Vol.40(12), 2021‑Dec
DOI:
10.1007/s00299-017-2202-4
PMCID:
4371917
Abstract
Here, we developed a reliable protocol for the fast and efficient gene-edited Anliu sweet orange plants production. The application of in vitro shoot grafting technology significantly reduced the growth cycle of transgenic seedlings, and the survival rate of cleft grafting was more than 90%. In addition, the mutation efficiency of the grafted geneedited sweet orange was significantly improved by short-term heat stress treatments. Thus, the combination strategy of grafting and heat stress treatments provided a reference for the fast and efficient multiplex gene editing of sweet orange.
Publication Types
Journal Article
Keywords
Efficient gene editing
Grafting
Heat stress
PTG/Cas9
Sweet orange
Grant Support
- the national key research and development program of china (): NO. 2018YFD1000101
- national natural science foundation of china (): NOs. 31925034
- national natural science foundation of china (): 31872052
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