Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9.
Authors: Zhao Chenglu, Lou Huanhuan, Liu Qian, Pei Siqi, Liao Qiansheng, Li Zhenghe
Journal: Journal of integrative plant biology (J Integr Plant Biol), Vol.66(10), 2024‑Oct
DOI:
10.1111/jipb.13741
Abstract
Tomato spotted wilt virus-mediated delivery of CRISPR/Cas9 bypasses the need for stable transformation and permits efficient, DNA-free genome editing in pepper. Remarkably, up to 77.9% of regenerated pepper plants contained heritable edits. This method has been validated with two pepper varieties and is compatible with existing tissue culture protocols.
Publication Types
Journal Article
Keywords
CRISPR/Cas9
Capsicum annuum
genome editing
pepper
regeneration
tomato spotted wilt virus
transient delivery
viral vector
Grant Support
- National Key Research and Development Program of China (): 2023YFD1400300
- National Key Research and Development Program of China (): 2022YFC2601000
- Postdoctoral Fellowship Program of CPSF (): GZC20241527
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